Bio-Rad伯乐 MiniOpticon双色实时荧光定量PCR
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- 简介:【介绍】 MiniOpticon实时PCR检测系统由高性能的MJ minicycler和紧凑的双色实时检测器组成;是较小的和较便携的实时PCR系统。 MiniOpticon 应用了在Opticon2系统上广泛使用的激发和检测技术,48个立的LED(发光二极管)阵列作为激发光源,顺序检测样品。每...
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【介绍】
MiniOpticon实时PCR检测系统由高性能的MJ minicycler和紧凑的双色实时检测器组成;是较小的和较便携的实时PCR系统。
MiniOpticon 应用了在Opticon2系统上广泛使用的激发和检测技术,48个立的LED(发光二极管)阵列作为激发光源,顺序检测样品。每一个LED的光束对准一个样品孔,保证较小的信号交叉和光的散射。LED每次点亮一个(48个样品,2色荧光检测,检测一次用时9秒)。顺序检测也能减少信号交叉。LED 光源寿命长,无需特别的维护。被激发出的发射波长信号进入2个带滤光片的光敏二极管(photodiode)进行检测。
整机没有在工作时运动的部件,保证定量检测和无需凝胶分析的可靠性。
主要特点
- 双通道检测
- 检测48个样品
- 仅重15lb,紧凑的外观(18 x 32 x 33 cm W x D x H)可以将仪器放置在任何地方
- 带有梯度的热循环系统,提供8个不同的温度条件,可以在同一个实验内优化反应条件
- 创新的48个LED阵列和光敏二极管,无需移动的部件
- 同一台电脑可以同时控制多大4台仪器。
【技术指标】
Base thermal cycler |
MJ Mini Cycler |
Sample capacity |
48-well PCR plate 6 x 8-tube strips |
Sample volume |
10–100 µl (20 µl recommended) |
Weight |
6.8 kg |
Dimensions (W x D x H) |
18 x 32 x 33 cm (7 x 12.5 x 13") |
Fluorescence excitation range |
470–500 nm |
Fluorescence detection ranges |
Channel 1: 523–543 nm
Channel 2: 540–700 nm |
|
Gradient Specifications |
Gradient range |
35–99°C |
Gradient accuracy |
±0.2°C of target at end columns within 30 sec (NIST–traceable) |
Temperature uniformity |
±0.4°C, in column, well-to-well, within 10 sec of target attainment |
Gradient calculator accuracy |
±0.4°C of actual column temperature (NIST–traceable) |
Temperature differential range |
1–16°C |
|
Recommended Computer Specifications |
Operating system |
Windows XP Professional |
Processor speed |
1.5 GHz |
Storage capacity |
20 GB hard drive |
Display |
1,024 x 768 pixel minimum screen resolution, 256 colors |
Memory |
512 MB RAM |
Note: Specifications subject to change without notice. |
【订货信息】
Accessories |
CFO-3202 |
Desktop Computer, includes 2.4 GHz processor, 256 MB RAM, 40 GB hard drive, 48 x 24 x 48 CD-RW, Windows XP Professional operating system |
CFO-3203 |
Laptop Computer, includes 2.4 GHz processor, 256 MB RAM, 40 GB hard drive, 24 x 10 x 24 CD-RW, Windows XP Professional operating system |
MiniOpticon Real-Time PCR Detection System |
CFB-3120 |
MiniOpticon Real-Time PCR System, includes optical housing, MJ Mini thermal cycler, analysis software |