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Bio-Rad伯乐 DCode通用突变检测系统

Bio-Rad伯乐 DCode通用突变检测系统

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  • 简介:【介绍】 系统功能灵活,适合多种突变检测方法 在癌症研究发展及多种单基因或多基因疾病的研究过程中,探索未知突变起着非常重要的作用。DNA 多态性 是物种的生态与进化研究非常有用的工具。应用范围包括物种鉴定、种群结构分析及遗传多样性研究等。 DCode...

询价/订购热线:400-098-6966  0371-60153588

 【介绍】
系统功能灵活,适合多种突变检测方法

在癌症研究发展及多种单基因或多基因疾病的研究过程中,探索未知突变起着非常重要的作用。DNA 多态性 是物种的生态与进化研究非常有用的工具。应用范围包括物种鉴定、种群结构分析及遗传多样性研究等。

DCode 系统由带温度控制模块(包括微处理器控制加热)的电泳设备、一个缓冲液再循环泵、一个搅拌器及 铺制特殊应用凝胶的附件组成。冷却槽及一个外部实验室冷却器可把运行温度控制在室温下。一块凝胶能在2 个小 时内运行64 个样品,并提供5-70°C 范围内的温度控制。它可与下列的电泳技术配合使用来检测单碱基变化:

  • 单链构象多态性(SSCP)
  • 变性梯度凝胶电泳(DGGE)
  • 时间温度梯度电泳(TTGE)
  • 均一变性凝胶电泳(CDGE)

为了获得较高的通量和效率,许多实验室采用多种技术。在快节奏研究环境中,不同方法间的快速切换和转换 到新技术的能力是非常关键的。DCode 系统能迎合主要突变检测技术的需要。其灵活的功能与优点包括:

  • 温度控制选择
  • 模块式设计,可满足用户现在与将来的需要
  • 的组合配件,简化启动过程
  • 针对技术的试剂和对照物,适合DGGE、CDGE、SSCP 和TTGE 等技术

 

 

系统功能灵活,适合多种突变检测方法

在癌症研究发展及多种单基因或多基因疾病的研究过程中,探索未知突变起着非常重要的作用。DNA 多态性 是物种的生态与进化研究非常有用的工具。应用范围包括物种鉴定、种群结构分析及遗传多样性研究等。

DCode 系统由带温度控制模块(包括微处理器控制加热)的电泳设备、一个缓冲液再循环泵、一个搅拌器及 铺制特殊应用凝胶的附件组成。冷却槽及一个外部实验室冷却器可把运行温度控制在室温下。一块凝胶能在2 个小 时内运行64 个样品,并提供5-70°C 范围内的温度控制。它可与下列的电泳技术配合使用来检测单碱基变化:

  • 单链构象多态性(SSCP)
  • 变性梯度凝胶电泳(DGGE)
  • 时间温度梯度电泳(TTGE)
  • 均一变性凝胶电泳(CDGE)

为了获得较高的通量和效率,许多实验室采用多种技术。在快节奏研究环境中,不同方法间的快速切换和转换 到新技术的能力是非常关键的。DCode 系统能迎合主要突变检测技术的需要。其灵活的功能与优点包括:

  • 温度控制选择
  • 模块式设计,可满足用户现在与将来的需要
  • 的组合配件,简化启动过程
  • 针对技术的试剂和对照物,适合DGGE、CDGE、SSCP 和TTGE 等技术

【技术指标】

 

【订货信息】

目录 # 描述
DCode Systems
170-9080 DCode System for DGGE, 120 V, for 16 cm gels with single prep well (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9081 DCode System for DGGE, 220/240 V, for 16 cm gels with single prep well (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9082 DCode System for DGGE, 100 V, for 16 cm gels with single prep well (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9083 DCode System for DGGE, 120 V, for 10 cm gels with 2 prep wells (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9084 DCode System for DGGE, 220/240 V, for 10 cm gels with 2 prep wells (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9085 DCode System for DGGE, 100 V, for 10 cm gels with 2 prep wells (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9086 DCode System for CDGE, 120 V, for 16 cm gels with 20 wells (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9089 DCode System for TTGE, 120 V, for 16 cm gels with 20 wells (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels, control reagents for TTGE
170-9090 DCode System for TTGE, 220/240 V
170-9091 DCode System for TTGE, 100 V
170-9092 DCode System for SSCP, 120 V, for 20 cm gels with 20 wells (0.75 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels, cooling tank adaptor for use with external cooling bath, control reagents for SSCP
170-9093 DCode System for SSCP, 220/240 V
170-9094 DCode System for SSCP, 100 V
170-9102 Complete DCode System, 120 V, Mac, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP systems
170-9103 Complete DCode System, 220/240 V, Mac, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP, plates, combs and spacers to cast 1 mm and 0.75 mm thick 10, 16, and 20 cm gels as described for the DGGE, CDGE, TTGE, and SSCP systems
170-9104 Complete DCode System, 100 V, Mac, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP systems
170-9105 Complete DCode System, 120 V, PC, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP, plates, combs and spacers to cast 1 mm and 0.75 mm thick 10, 16 and 20 cm gels as described for the DGGE, CDGE, TTGE, and SSCP systems
170-9106 Complete DCode System, 220/240 V, PC, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP, plates, combs and spacers to cast 1 mm and 0.75 mm thick 10, 16, and 20 cm gels as described for the DGGE, CDGE, TTGE, and SSCP systems
170-9107 Complete DCode System, 100 V, PC, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP, plates, combs and spacers to cast 1 mm and 0.75 mm thick 10, 16, and 20 cm gels as described for the DGGE, CDGE, TTGE, and SSCP systems
DCode Adaptor Kits
170-9125 DGGE Kit, for 16 cm gels with single prep well (1 mm), includes sandwich clamps, pressure clamp, comb gasket and holder, fittings required for gradient gel casting
170-9126 DGGE Kit, for 10 cm gels with 2 prep wells (1 mm), includes sandwich clamps, pressure clamp, comb gasket and holder, fittings required for gradient gel casting
170-9127 CDGE/TTGE Kit, for 16 cm gels with 20 wells (1 mm), includes sandwich clamps, pressure clamp, comb gasket and holder, fittings required for gradient gel casting
170-9128 Complete SSCP Kit, for 20 cm gels with 20 wells (0.75 mm), includes sandwich clamps, cooling finger adaptor for use with external chiller
170-9129 Basic SSCP Kit, for 20 cm gels with 20 wells (0.75 mm), includes sandwich clamps
DCode Accessories
170-9034 MacMelt Software, Mac
170-9042 Model 475 Gradient Delivery System, includes cam-operated manual gradient former, 2 each of 10 and 30 ml syringes, all accessories required to cast gradient gels
170-9140 Electrophoresis Cooling Tank, with cooling adaptor for hook-up to laboratory recirculating chiller
170-9240 WinMelt Software, PC/Windows
Electrophoresis Reagents and DNA Control Reagents
170-9150 DCode Control Reagent Kit for DGGE/CDGE/TTGE, includes primers (one GC-clamped) and DNA templates for production of wild-type and mutant DNA
170-9151 DCode Control Reagent Kit for SSCP, includes primers and DNA templates for production of wild-type and mutant DNA
170-9170 DCode Electrophoresis Reagent Kit for DGGE, includes 500 ml 40% acrylamide/bis (37.5:1), 2 x 1 L 50x TAE buffer, 225 ml *** deionized formamide, 10 ml 10 mg/ml ethidium bromide, 10 ml DCode dye solution, 5 ml TEMED, 1 ml 2x gel loading dye, 10 g ammonium persulfate
170-9172 DCode Electrophoresis Reagent Kit for SSCP, includes 500 ml 40% acrylamide solution, 500 ml 2% bis-acrylamide solution, 100 ml glycerol, 6 x 1 L 10x TBE buffer, 2x SSCP gel loading dye, 5 ml TEMED, 10 g ammonium persulfate

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