CHEF Mapper XA 系统为你提供2 种途径 以优化分离。特的自动计算功能整合了11 个关键变量及设定起始分离条件，能为你的分 离自动选择条件。以Windows 为基础的交互式运算程序可用于改进实验流程。程序能让你同时确定多个电泳变量以得出较优化的分离规程。

繁忙的实验室需要能储存关键分离条件的设备。CHEF Mapper XA 系统能储存多达99 个程序。

CHEF Mapper XA 系统是较灵活的PFGE 单元。与其它PFGE 系统相比，CHEF Mapper XA 系统可获得更高的分辨率、更快的速度和更高的度，是PFGE 应用的理想选择。

CHEF Mapper XA 系统能让你选择0°–360° 内的任何脉冲角度，使你用一个系统就能完成染色体和质粒DNA 的分离。

的片段大小分离需要一个扩展的线性分离跨度。通过逐渐改变电泳过程中的转换时间来转换时间梯度，从而增加片段迁移率。非线性（例如，凹形或凸形）梯度改变电泳起始到结束过程中的转换时间增量。非线性梯度可线性分离50–700 kb 片段，获得更的片段大小。

CHEF Mapper XA 系统的多阶段模式可在选定的大小跨度内提高片段分辨率。 每个载体（脉冲角度）均可电压（场强度）及转换时间（脉冲持续时间）。一个 电泳较多能融合8 个不同的阶段，以优化样品中亚组分片段的分离。确定电压、持续时间、角度和频率的二次脉冲应用能提高DNA 分子的分离和分辨率。二次脉 冲能释放被凝胶基质捕获的DNA。

 

 

左，2 阶段模式，24 小时电泳，120° 角度.  60-120秒转换时间梯度，  6 V/cm（200 V）, 0.5 x TBE, 14°C,  1.0% 脉冲场Certified 琼脂糖  右，多阶段模式，60 小时电泳阶段（脉冲角度）：  1. 90 秒转换时间， –60° 角度  2. 45 秒转换时间，180° 角度  3. 90 秒转换时间，60° 角度  4. 90 秒转换时间，–60° 角度  5. 90 秒转换时间，60° 角度  6. 45 秒转换时间，180° 角度  7. 90 秒转换时间，–60° 角度  8. 90 秒转换时间，60° 角度

 

 

多阶段模式，20 小时电泳，120°角度，  60–120 秒转换时间梯度，  6 V/cm（200 V）, 0.5 x TBE, 14°C,  1% Certified 分子生物琼脂糖  二次脉冲  6 V/cm（200 V），0 角度，  3 秒转换时间，  4 脉冲/分钟

 

 

目录 #	描述
CHEF Mapper Systems
170-3670	CHEF Mapper XA Chiller System, 120 V, includes CHEF Mapper XA power module, embedded auto-algorithm for protocol optimization, interactive algorithm program disk, electrophoresis cell, cooling module, variable-speed pump, Tygon tubing (12'), 14 x13 cm (W x L) casting stand, 15-well 1.5 mm comb and comb holder, screened cap, disposable plug molds, leveling bubble, cables, S. cerevisiae standards, two 0.5 A FB fuses, 5 g pulsed field certified agarose, 5 g Certified megabase agarose, instructions
170-3671	CHEF Mapper XA Chiller System, 100 V
170-3672	CHEF Mapper XA Chiller System, 220 V
170-3673	CHEF Mapper XA Chiller System, 240 V, includes CHEF Mapper XA power module, embedded auto-algorithm for protocol optimization, interactive algorithm program disk, electrophoresis cell, cooling module, variable-speed pump, Tygon tubing (12'), 14 x13 cm (W x L) casting stand, 15-well 1.5 mm comb and comb holder, screened cap, disposable plug molds, leveling bubble, cables, S. cerevisiae standards, two 0.5 A FB fuses, 5 g pulsed field certified agarose, 5 g Certified megabase agarose, instructions
Accessories
162-0196	Zeta-Probe GT Membrane, 30 cm x 3.3 m, 1 roll
162-0197	Zeta-Probe GT Membrane, 20 cm x 3.3 m, 1 roll
170-3622	Reusable Plug Mold, 10 plug
170-3623	Preparative Comb, 14 cm wide, 1.5 mm thick, with 2 outer wells for size standards
170-3627	15-Well Comb, 21 cm wide, 1.5 mm thick
170-3628	30-Well Comb, 21 cm wide, 1.5 mm thick
170-3643	Gel Scoop
170-3644	Variable-Speed Pump, 120 V
170-3645	45-Well Comb, 21 cm wide, 1.5 mm thick
170-3648	Electrodes, thick gauge (0.02"), 6
170-3654	Cooling Module, 120 V
170-3655	Cooling Module, 220/240 V
170-3688	Cooling Module, 100 V
170-3689	Standard Casting Stand, includes 14 x 13 cm frame and platform
170-3699	Combination Comb Holder
170-3704	Wide/Long Combination Casting Stand, includes 21 x 14 cm frame and platform
170-3711	Screened Caps, 5
170-3713	50-Well Disposable Plug Molds, 5
170-4046	Leveling Table, 20 x 30 cm
170-4322	20-Well Comb, 14 cm wide, 1.5 mm thick, adjustable height
170-4323	15-Well Comb, 14 cm wide, 0.75 thick, adjustable height
170-4324	15-Well Comb, 14 cm wide, 1.5 mm thick, adjustable height
170-4325	10-Well Comb, 14 cm wide, 0.75 mm thick, adjustable height
170-4326	10-Well Comb, 14 cm wide, 1.5 mm thick, adjustable height
170-4344	30-Well Comb, 14 cm wide, 1.5 mm thick, adjustable height
Agaroses and Size Standards
162-0017	Low Melt Preparative Grade Agarose, 25 g
162-0019	Low Melt Preparative Grade Agarose, 100 g
162-0135	Chromosomal Grade Agarose, 25 g
162-0136	Chromosomal Grade Agarose, 100 g
162-0137	Pulsed Field Certified Agarose, 100 g
162-0138	Pulsed Field Certified Agarose, 500 g
170-3592	CHEF Bacterial Genomic DNA Plug Kit, contains 12 ml cell suspension buffer, 1.3 ml proteinase K, 30 ml proteinase K reaction buffer, 12 ml 2% CleanCut agarose, 60 ml 10x wash buffer, 1.6 ml lysozyme (25 mg/ml), 30 ml lysozyme buffer, screened cap, 2 disposable plug molds, instructions
170-3593	CHEF Yeast Genomic DNA Plug Kit, contains 12 ml cell suspension buffer, 1.3 ml proteinase K, 30 ml proteinase K reaction buffer, 12 ml 2% CleanCut agarose, 60 ml 10x wash buffer, 1.6 ml lyticase, 25 ml lyticase buffer, screened cap, 2 disposable plug molds, instructions
170-3605	CHEF DNA Size Marker, S. cerevisiae, 0.2–2.2 Mb, 5 agarose blocks, sufficient for 25–40 plugs
170-3624	CHEF DNA Size Standard, 5 kb ladder, 24–40 applications
170-3633	CHEF DNA Size Marker, S. pombe, 3.5–5.7 Mb, 5 agarose blocks, sufficient for 25–40 plugs
170-3635	CHEF DNA Size Standard, lambda ladder, 0.05–1 Mb, 5 agarose blocks, sufficient for 25–40 plugs
170-3667	CHEF DNA Size Marker, H. wingei, 1–3.1 Mb, 5 agarose blocks, sufficient for 25–40 plugs
170-3707	CHEF DNA Size Standard, 8–48 kb, 125 lanes