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bio-rad伯乐 731-8300,731-8301 BioLogic LP层析系统

bio-rad伯乐 731-8300,731-8301 BioLogic LP层析系统

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  • 简介:BioLogic LP 低压层析系统是生物分子纯化的理想选择。它具有功能多、使用简便、经济等特点。小巧的设 计较大限度地节约冷库和实验室的工作空间。BioLogic LP 系统出色的性能如下: LP Data View 软件 专为BioLogic LP 系统设计的Lp Data View 软件使用简便;...

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 BioLogic LP 低压层析系统是生物分子纯化的理想选择。它具有功能多、使用简便、经济等特点。小巧的设 计较大限度地节约冷库和实验室的工作空间。BioLogic LP 系统出色的性能如下:
  • LP Data View 软件— 专为BioLogic LP 系统设计的Lp Data View 软件使用简便;可完成数据采集等多重任务,只需一个串行端口即可对任何Windows95, 98,Me, NT4.0 或2000 操作系统的数据进行打印 
     
  • 方法程序储存— 储存50 个方法程序;每种方法包括多达50 个泵操作步骤和50 个组分收集步骤 
     
  • 缓冲液选择— 可选择5 种缓冲液,加入SV–5 液流选择阀可进行全自动样品分离和大体积样品输入 
     
  • 检测性能— 含有检测蛋白和核酸的滤光片(280nm 和254nm),以及检测梯度进程的电导检测器 
     
  • 高流速泵— 系统配置了一个流速范围0.05–40 ml/min 较大背压30 psi 的蠕动泵。系统可兼容Econo–Column 低压层析柱、Macro–Prep 层析介质、Econo–Pac 滤柱、Amersham HiTrap 滤柱、SOURCE 介质和其它低压层析介质 
     
  • 组份收集— 系统有简单组分和复杂组分两种收集选择: 2110 型组分收集器收集至80 根13×100 毫米试管或离心管中,或更的BioFrac 组分收集器收集至酶标板、细口大玻璃瓶等任意容器中。同时,BioLogicLP 系统支持许多非Bio–Rad 组分收集器

BioLogic LP Data View 软件

操作简单的LP Data View 软件可把任何能运行Windows 的计算机转变成一个记录仪。但与普通的记录仪 相比,具有更为灵活的功能。它可自由调节运行中或运行后的量程,在捕获层析数据时执行多项工作,并能通 过任何或网络打印机进行打印。用户会感到储存和获取数据比以往任何时候更轻松。LP Data View 软 件的功能如下:

  • 运行于Windows 95, 98, ME, NT4.0 版或2000 操作系统;需要使用一个串行端口
  • 自动记录每次操作的运行信息,同时记录数据与注释
  • 自动记录运行过程如启动、结束、组分收集器移动,等待,暂停和继续
  • 运行数据保存于立的数据文件
  • 打印用户报告
  • 数据输出

需要LP Data View 软件和BioLogic LP 系统的演示,请与当地Bio–Rad 销售代 表联系。


 

1327 型记录仪

1327 型记录仪是一款设计精巧的双笔记录仪,为空间局促的实验室和冷 库的理想选择。它能与许多检测装置兼容。与Econo 蠕动泵、BioLogic LP、 或BioLogic DuoFlow 系统连接时,能自动完成记录笔的起放,走纸和操作 标记等功能。主要特征包括:

  • 双通道,双笔配置
  • 彩色记录装置,与Econo 蠕动泵, BioLogic LP 系统和BioLogic DuoFlow系统匹配
  • 1 mV–20 V 电压范围,适合任何紫外/可见、pH、折光率或电导率检测器
  • 机械的刻度偏置管,对记录笔到达到纸顶时非常有用
  • 12 种走纸速率,以秒、分、小时或天为单位记录数据


 

BioLogic LP 阀系统

液流选择和自动加样阀
SV–5 液流选择阀拓展了BioLogic LP 系统制备纯化的性能,可选择性控制5 种缓冲液和自动输入大体积样品。 SV–5 阀直接安置于BioLogic 层析架上,由BioLogic LP 系统调控。

人工进样阀
MV–6 进样阀有6 个带有凹luer 接头的口。它能与任何容积的自制上样环匹配。MV–6 阀直接安置于BioLogic 系统层析架和Econo 梯度泵支架上。

组分收集/层析柱旁通阀
SV–3 分流/旁通阀是由Econo 梯度泵、EP–1 型Econo 泵或BioLogic LP 系统控制的二相螺线管阀。当与 BioLogic LP 系统连接时,SV–3 阀将液体从层析柱导入 至旁通位点,或至组分收集器或至废液位点。阀的功 能由后置的mini–DIN 接头和阀管线决定。当与 Econo 泵或Econo 梯度泵连接,SV–3 阀只作为组分收集器分流 阀使用。

SV–3 阀直接与BioLogic 系统层析架和Econo 梯度泵支架连接。


 

Econo 梯度组合泵

特的Econo 梯度泵适用于任何低压蛋白纯化系统。它是能立应用于均一洗脱和 梯度洗脱的蠕动泵。Econo 梯度泵是一个双通道双向泵,应用于制备层析或重组蛋白亲和 层析纯化。Econo 梯度泵可与2110 型、BioFrac 组分收集器,EM–1 型Econo 紫外检 测器、EG–1 型Econo 梯度检测器和1327 型记录仪等设备匹配。该Econo 梯度 组合泵是您经济而实用的*选。

开发新的应用领域
  • 串联收集— 将层析柱流出液分流,收集至两个不同部位的试管或微孔板中,作进一部的测试和分析
  • 取样操作— 发酵罐在发酵进程中可例行取样,以监控目标产品的浓度;培养液通过Econo 梯度泵再循环,分流阀定时将等量试样送至组分收集器作进一步分析
  • 实时生物活性检测— 流出液中添加底物来检测反应混合物。Econo 梯度泵可提供一定流速的底物,并通过三 通管与分装的流出液混合。收集反应混合液作进一步 分析,或送至流动池记录生色团的变化
梯度泵具有以下优点:
  • 每个通道的流速0.002–40ml/min (每泵2 个通道,较高流速达20ml/min );分装阀组合套件流速可达> 2μl/min
  • 控制二元梯度混合器
  • 安装、操作简便
  • 时间和体积两种梯度控制模式
  • 控制分流阀收集废物或组分(尤其适合较大体积的分子量排阻层析)
  • 控制组分收集器的启动/停止;一些组分收集器具有“等待”和“暂停”功能
  • 控制记录仪的启动/停止,记录笔的起/落
  • 控制分装阀的百分比增量
  • 根据不同规格管线自动调节收集程序
  • 经济、简便、快速连接的Luer–LOK 接头
  • 液晶显示器对比度可调,的记数器确认管线磨损程度,有效按键蜂鸣声
  • 节省空间的整合设计
  • 与BioLogic DuoFlow 层析系统兼容

【技术指标】

Pump  
   Flow range 0.1–40 ml/min
   Maximum counterpressure 30 psi (2 bar)
   Gradient formation Solenoid-activated proportioning valve
Mixer volume: 750 µl
Detection Performance  
   UV Single channel, 280/254 nm with auto zero
Detection range: 0.001–2 AUFS
   Conductivity Detection range: 0.5–500 mS/cm FS
Temperature Range 4–40°C
Sample Loading Manual loop fill
Automatic loading through SV-5 buffer select valve
Technical Specifications BioLogic DuoFlow Systems BioLogic LP System Econo Gradient Pump Kits
Flow rate limit 40 ml/min; optional F40 pump kit
80 ml/min; optional Maximizer valve system
20 ml/min per channel; dual-channel peristaltic pump 20 ml/min per channel; dual-channel peristaltic pump
Pressure limit 3,500 psi (23 MPa) at 10 ml/min
1,000 psi (6.6 MPa) at 40 ml/min
30 psi (0.2 MPa) 30 psi (0.2 MPa)
UV detection Standard 254 and 280 nm filters; optional 214, 365, 405, 436, and 546 nm filters; standard 5 mm analytical flow cell; optional 2 mm preparative flow cell; optional BioLogic QuadTec UV/Vis detector, 4-wavelength simultaneous detection 254 and 280 nm filters; 2 mm flow cell Optional Model EM-1 Econo UV monitor; 254 and 280 nm filters
Conductivity Standard Standard Optional Model EG-1 Econo gradient monitor
pH detection Optional N/A N/A
Other detectors Optional signal import module (SIM), adds pH, refractive index, and fluorescence detectors N/A N/A
Buffer selection/large sample loading Optional SV5-4 buffer-select valve, Model EP-1 Econo pump or Econo gradient pump Optional SV-5 buffer-select valve, Model EP-1 Econo pump or Econo gradient pump Optional SV3-2 valve
Sample injection AVR7-3 valve Manual MV-6 valve Optional MV-6 valve
Multiple sample injection Optional DynaLoop sample loops; 25 ml, 90 ml N/A N/A
General fraction collection BioFrac or Model 2110 fraction collector BioFrac or Model 2110 fraction collector BioFrac or Model 2110 fraction collector
Large-volume fraction collection BioFrac fraction collector with optional prep adaptor or AVR9-8 valve BioFrac fraction collector with optional prep adaptor BioFrac fraction collector with optional prep adaptor
Small-volume fraction collection BioFrac or Model 2110 fraction collector BioFrac or Model 2110 fraction collector BioFrac or Model 2110 fraction collector
Buffer blending Optional Maximizer valve system N/A N/A
Column scouting/column switching Optional AVR9-8 valves for ≤8 columns or additional AVR7-3 valve for 2 columns N/A N/A
Reverse-flow chromatography Additional AVR7-3 valve N/A N/A
Solvent compatibility All commonly used chromatographic solvents All commonly used chromatographic solvents All commonly used chromatographic solvents
Operating temperature 4–40°C 4–40°C 4–40°C
Dimensions (W x D x H) 35 x 41 x 81 cm 
(13.8 x 16 x 31.8")
24 x 33 x 25 cm 
(9.5 x 13 x 9.8")
14 x 20 x 20 cm 
(5.5 x 7.9 x 3.9")
Weight 70 kg (154 lb) 19 kg (42 lb) 3.5 kg (7.7 lb)

【订货信息】

目录 # 描述
BioLogic LP Systems
731-8300 Standard BioLogic LP System, 100/120 V, includes BioLogic LP controller, BioLogic rack, accessory kit with inject valve (MV-6), proportioning valve/mixer, UV optics, conductivity cell, tubing and fittings kit, column and conductivity cell holder, starter kit, instructions
731-8301 Standard BioLogic LP System, 220/240 V, includes BioLogic LP controller, BioLogic rack, accessory kit with inject valve (MV-6), proportioning valve/mixer, UV optics, conductivity cell, tubing and fittings kit, column and conductivity cell holder, starter kit, instructions
BioLogic LP Systems With Fraction Collector
731-8302 BioLogic LP System With Model 2110 Fraction Collector, 100/120 V, includes standard BioLogic LP system, SV-3 diverter/bypass valve, system cable 1
731-8303 BioLogic LP System With Model 2110 Fraction Collector, 220/240 V, includes standard BioLogic LP system, SV-3 diverter/bypass valve, system cable 1
731-8304 BioLogic LP System With BioFrac Fraction Collector, 100/120 V, includes standard BioLogic LP system, system cables 3 and 15
731-8305 BioLogic LP System With BioFrac Fraction Collector, 220/240 V, includes standard BioLogic LP system, system cables 3 and 15
BioLogic LP System With Fraction Collector and Chart Recoder
731-8306 BioLogic LP System With Model 2110 Fraction Collector and Model 1327 Chart Recorder, 100/120 V, includes BioLogic standard system, Model 2110 fraction collector, Model 1327 chart recorder, SV-3 diverter/bypass valve, system cables 1, 2, and 4
731-8307 BioLogic LP System With Model 2110 Fraction Collector and Model 1327 Chart Recorder, 220/240 V, includes BioLogic standard system, Model 2110 fraction collector, Model 1327 chart recorder, SV-3 diverter/bypass valve, system cables 1, 2, and 4
731-8308 BioLogic LP System With BioFrac Fraction Collector and Model 1327 Chart Recorder, 100/120 V, includes BioLogic standard system, BioFrac fraction collector, Model 1327 chart recorder, system cables 2, 3, 4, and 15
731-8309 BioLogic LP System With BioFrac Fraction Collector and Model 1327 Chart Recorder, 220/240 V, includes BioLogic standard system, BioFrac fraction collector, Model 1327 chart recorder, system cables 2, 3, 4, and 15
BioLogic LP Systems With Fraction Collector and BioLogic LP Data View Software
731-8336 BioLogic LP System With Model 2110 Fraction Collector and LP Data View Software, 110/120 V, includes SV-3 diverter/bypass valve, system cable 1, 25' serial cable
731-8337 BioLogic LP System With Model 2110 Fraction Collector and LP Data View Software, 220/240 V, includes SV-3 diverter/bypass valve, system cable 1, 25' serial cable
731-8338 BioLogic LP System With BioFrac Fraction Collector and LP Data View Software, 110/120 V, includes system cables 3 and 15, 25' serial cable
731-8339 BioLogic LP System With BioFrac Fraction Collector and LP Data View Software, 220/240 V, includes system cables 3 and 15, 25' serial cable
BioLogic LP Accessories and Replacement Parts
731-8155 Conductivity Flow Cell
731-8320 MV-6 Sample Inject Valve, 6 ports
731-8321 SV-5 Buffer Select Valve, 5-port, 4-position solenoid random-access valve, 30 psi (2 bar) limit
731-8322 SV-3 Diverter/Bypass Valve, 3-way valve
731-8323 Gradient Mixer
731-8324 BioLogic LP Optics Module
731-8350 BioLogic LP Starter Kit, includes buffers, standard, anion exchange cartridge, instructions

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