【介绍】
PROTEAN Plus Dodeca 高通量电泳槽能容纳12 块大型板式凝胶,与PROTEAN IEF等电聚 焦电泳系统的高通量匹配,满足双向电泳高容量 的应用要求*。PROTEAN Plus Dodeca 高通量电 泳槽的冷却、再循环和板式电极等特点能提供稳 定均一的高分辨率结果。PROTEAN Plus Dodeca 高通量电泳槽有以下特点:
- 可同时进行1-12 块凝胶电泳,满足高通量和可 重复的要求
- 陶瓷冷却芯、缓冲液再循环泵**和外接冷却循环 器能提供高分辨结果所需的有效冷却——使得凝 胶周围缓冲液温度变化小于1 °C
- 板式电极**能产生较均一的电场,保证样品在同 一条件下进行电泳。
- 可适用于多种凝胶规格:18.3 x 19.3 cm(带多歧管)、 20 x 20.5 cm 或25 x 20.5 cm (W x L),厚度1.0、1.5 或2.0 mm
- 带有封边垫条的合页式的玻板(hinged spacer plates)设计,保证对位,避免漏电。
- 玻板有多种高度便于IPG 胶条或管式凝胶上样:AnyGel 置胶架可在上样过程中稳定和放置凝胶。
- 有多种专为Dodeca 电泳槽设计的消耗品,使用更为方便。详见PROTEAN Plus 预制胶, PROTEAN Plus 覆盖琼制糖,分子量Precision Plus Protein 蛋白标准品插块。
* IPG 和管式凝胶样品专为电泳而设计;不推荐用于1-D 应用。
** 美国***6,451,193
【技术指标】
凝胶数量 |
1–12块 |
预制胶 |
PROTEAN Plus Ready Gel 预制胶 |
手灌胶 |
用PROTEAN Plus 合页式的玻板和
PROTEAN Plus 多板凝胶凝胶灌胶器灌制 |
典型电泳条件 |
12 块手灌凝胶,200 V 恒压, ~800 mA (初始) 电泳6-7 小时 |
推荐电源 |
PowerPac HC 或PowerPac 通用电源 |
冷却系统 |
内置陶瓷冷却芯,外围缓冲液再循环泵和外接冷却循环器
(循环器需另行购买;推荐流速10-15 gal/min,
推荐冷却功率>250 W , 20°C) |
所需的总缓冲液体积 |
22.5 L |
大小(W x L x H) |
槽:34.5 x 39.5 x 34.5 cm; 泵: 17.5 x 19.5 x 12 cm |
【订货信息】
PROTEAN Plus Dodeca Cell |
165-4150 |
PROTEAN Plus Dodeca Cell, 100/120 V, includes electrophoresis buffer tank with built-in ceramic cooling core, lid, buffer recirculation pump with tubing, 2 gel releasers, instructions |
165-4151 |
PROTEAN Plus Dodeca Cell, 220/240 V, includes electrophoresis buffer tank with built-in ceramic cooling core, lid, buffer recirculation pump with tubing, 2 gel releasers, instructions |
165-4158 |
Recirculation Pump, 100/120 V |
165-4159 |
Recirculation Pump, 220/240 V |
Replacement Parts |
165-4153 |
Replacement Tubing Kit, for tank with stopcock drain port installed at base of tank |
165-4154 |
Replacement Gasket Assembly |
165-4155 |
Replacement Electrode Card, anode |
165-4156 |
Replacement Electrode Card, cathode |
165-4157 |
Replacement Lid |
165-4166 |
Manifold Tubing, required for precast gels and PROTEAN II plates, 11 pieces |
165-2948 |
Replacement Power Cables, for lid |
165-4167 |
Buffer Exhaust Tubing |
PROTEAN Plus Hinged Cassettes |
165-4170 |
Hinged Spacer Plates, for 20 x 20.5 cm (W x L) gels, 1.0 mm, 1 set |
165-4171 |
Hinged Spacer Plates, for 20 x 20.5 cm (W x L) gels, 1.5 mm, 1 set |
165-4172 |
Hinged Spacer Plates, for 20 x 20.5 cm (W x L) gels, 2.0 mm, 1 set |
165-4173 |
Hinged Spacer Plates, for 25 x 20.5 cm (W x L) gels, 1.0 mm, 1 set |
165-4174 |
Hinged Spacer Plates, for 25 x 20.5 cm (W x L) gels, 1.5 mm, 1 set |
165-4175 |
Hinged Spacer Plates, for 25 x 20.5 cm (W x L) gels, 2.0 mm, 1 set |
Accessories |
165-3320 |
Gel Releasers, 5 |
165-4121 |
Model 495 Gradient Former, 100–1,500 ml, includes body with valve stem and tubing connection kit |
165-4160 |
PROTEAN Plus Multi-Casting Chamber, includes casting chamber, sealing plate, silicone gasket, tapered luer connector, leveling bubble, 8 acrylic blocks, 15 separation sheets, iinstructions (order glass hinged spacer plates and combs separately) |
165-5131 |
AnyGel Stand, 6-row, holds 6 PROTEAN gels, 12 Criterion gels, or 18 ReadyGel mini gels; includes instructions |
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